Increased contractile strength and tightened adhesions to the substratum result from reverse transformation of cho cells by dibutyryl cyclic adenosine monophosphate | Zendy

W. Mark Leader | Zendy; David Stopak | Zendy; Albert K. Harris | Zendy

Open Access

Increased contractile strength and tightened adhesions to the substratum result from reverse transformation of cho cells by dibutyryl cyclic adenosine monophosphate

Author(s) -

W. Mark Leader,

David Stopak,

Albert K. Harris

Publication year - 1983

Publication title -

journal of cell science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.384

H-Index - 278

eISSN - 1477-9137

pISSN - 0021-9533

DOI - 10.1242/jcs.64.1.1

Subject(s) - biology , contractility , adhesion , adenosine , morphology (biology) , microbiology and biotechnology , transformation (genetics) , bucladesine , biophysics , endocrinology , biochemistry , intracellular , materials science , composite material , genetics , gene

We have examined the changes in cellular contractility and adhesive morphology that accompany the reverse transformation of CHO cells caused by dibutyryl cyclic adenosine monophosphate. The ability of these cells to distort elastic silicone rubber substrata was found to increase markedly over the 5 h period following addition of cyclic AMP and testosterone to the medium, and to relax again following hormone removal. Parallel changes were observed in the interference reflexion image of the adhesion of these cells to glass substrata; broad grey-appearing 'close' contacts characteristic of the transformed state were gradually replaced by numerous small black-appearing 'focal contacts'. These changes in adhesive morphology required considerably longer (20-25 h) to be completed or to revert, however.

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