Open AccessLamin regulates the dietary restriction response via the mTOR pathway in Caenorhabditis elegans
Author(s) -
Chayki Charar,
Sally Metsuyanim-Cohen,
Daniel Z. Bar
Publication year - 2021
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.258428
Subject(s) - biology , caenorhabditis elegans , pi3k/akt/mtor pathway , p70 s6 kinase 1 , mtorc1 , lamin , regulator , downregulation and upregulation , microbiology and biotechnology , activator (genetics) , rptor , gene , genetics , signal transduction
Animals subjected to dietary restriction (DR) have reduced body size, low fecundity, slower development, lower fat content and longer life span. We identified lamin as a regulator of multiple dietary restriction phenotypes. Downregulation of lmn-1 , the single Caenorhabditis elegans lamin gene, increased animal size and fat content specifically in DR animals. The LMN - 1 protein acts in the mTOR pathway, upstream of RAPTOR and S6 kinase β1 (S6K), a key component of and target of the mechanistic target of rapamycin (mTOR) complex 1 (mTORC1), respectively. DR excludes the mTORC1 activator RAGC-1 from the nucleus. Downregulation of lmn-1 restores RAGC-1 to the nucleus, a necessary step for the activation of the mTOR pathway. These findings further link lamin to metabolic regulation.