Open AccessAn indispensable role for dynamin-related protein 1 (DRP1) in beige and brown adipogenesis
Author(s) -
Raja Gopal Reddy Mooli,
Dhanunjay Mukhi,
Zhonghe Chen,
Nia Buckner,
S Ramakrishnan
Publication year - 2020
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.247593
Subject(s) - adipogenesis , biology , microbiology and biotechnology , prdm16 , dnm1l , mitochondrial fission , mitochondrial biogenesis , adipocyte , mitochondrion , adipose tissue , mitochondrial fusion , brown adipose tissue , mitochondrial dna , endocrinology , genetics , gene , mesenchymal stem cell
Emerging evidence indicates that proper mitochondrial dynamics is critical for adipocyte differentiation and functional thermogenic capacity. We found that mitochondrial fission protein dynamin-related protein 1 (DRP1) is highly expressed in brown adipose tissue compared to white adipose tissue and their levels increase during brown adipocyte differentiation. Our results reveal that the inhibition of DRP1 using Mdivi-1 mitigates beige adipocyte differentiation and differentiation-associated mitochondrial biogenesis. We found that DRP1 is essential for the induction of the early phase beige adipogenic transcriptional program. Intriguingly, inhibition of DRP1 is dispensable following the induction of beige adipogenesis and adipogenesis-associated mitochondrial biogenesis. Together, we demonstrate that DRP1 in the preadipocytes plays an essential role in beige and brown adipogenesis.