Open AccessMyosin 1b flattens and prunes branched actin filaments
Author(s) -
Julien Pernier,
Antoine Morchain,
Valentina Caorsi,
Aurélie Bertin,
Hugo Bousquet,
Patricia Bassereau,
Evelyne Coudrier
Publication year - 2020
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.247403
Subject(s) - biology , cofilin , actin , myosin , microbiology and biotechnology , actin remodeling , tropomyosin , total internal reflection fluorescence microscope , profilin , biophysics , actin cytoskeleton , cytoskeleton , biochemistry , membrane , cell
Motile and morphological cellular processes require a spatially and temporally coordinated branched actin network that is controlled by the activity of various regulatory proteins, including the Arp2/3 complex, profilin, cofilin and tropomyosin. We have previously reported that myosin 1b regulates the density of the actin network in the growth cone. Here, by performing in vitro F-actin gliding assays and total internal reflection fluorescence (TIRF) microscopy, we show that this molecular motor flattens (reduces the branch angle) in the Arp2/3-dependent actin branches, resulting in them breaking, and reduces the probability of new branches forming. This experiment reveals that myosin 1b can produce force sufficient enough to break up the Arp2/3-mediated actin junction. Together with the former in vivo studies, this work emphasizes the essential role played by myosins in the architecture and dynamics of actin networks in different cellular regions.This article has an associated First Person interview with the first author of the paper.