Open AccessStaufen1 localizes to the mitotic spindle and controls the localization of RNA populations to the spindle
Author(s) -
Sami Hassine,
Florence Bonnet-Magnaval,
Louis Philip Benoit Bouvrette,
Bellastrid Doran,
Mehdi Ghram,
Mathieu Bouthillette,
Éric Lécuyer,
Luc DesGroseillers
Publication year - 2020
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.247155
Subject(s) - biology , spindle apparatus , spindle pole body , mitosis , microbiology and biotechnology , rna , kinetochore , genetics , cell division , gene , cell , chromosome
Staufen1 (STAU1) is an RNA-binding protein involved in the posttranscriptional regulation of mRNAs. We report that a large fraction of STAU1 localizes to the mitotic spindle in the colorectal cancer HCT116 and in the non-transformed hTERT-RPE1 cells. Spindle-associated STAU1 partly co-localizes with ribosomes and active sites of translation. We mapped the molecular determinant required for STAU1/spindle association within the first 88 N-terminal amino acids, a domain that is not required for RNA binding. Interestingly, transcriptomic analysis of purified mitotic spindles reveals that 1054 mRNAs as well as the precursor ribosomal RNA and lncRNAs and snoRNAs involved in ribonucleoprotein assembly and processing are enriched on spindles compared to cell extracts. STAU1 knockout causes the displacement of the pre-rRNA and of 154 mRNAs coding for proteins involved in actin cytoskeleton organization and cell growth, highlighting a role for STAU1 in mRNA trafficking to spindle. These data demonstrate that STAU1 controls the localization of sub-populations of RNAs during mitosis and suggests a novel role of STAU1 in pre-rRNA maintenance during mitosis, ribogenesis and/or nucleoli reassembly.