Open AccessSec71 separates Golgi stacks in Drosophila S2 cells
Author(s) -
Syara Fujii,
Kenji Kurokawa,
Tatsuya Tago,
Ryota Inaba,
Arata Takiguchi,
Akihiko Nakano,
Takunori Satoh,
Akiko Satoh
Publication year - 2020
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.245571
Subject(s) - biology , golgi apparatus , drosophila (subgenus) , microbiology and biotechnology , evolutionary biology , genetics , endoplasmic reticulum , gene
Golgi stacks are the basic structural units of the Golgi apparatus. Golgi stacks are separated from each other and scattered in the cytoplasm of Drosophila cells. Here, we report that the ARF-GEF inhibitor Brefeldin A (BFA) induces the formation of BFA bodies, which are aggregates of Golgi stacks, trans-Golgi networks, and recycling endosomes. Recycling endosomes are located in the centers of BFA bodies, while Golgi stacks surround them on their trans sides. Live imaging of S2 cells revealed that Golgi stacks repeatedly merged and separated on their trans sides, and BFA caused successive merger by inhibiting separation, forming BFA bodies. S2 cells carrying genome-edited BFA-resistant mutant Sec71M717L did not form BFA bodies at high concentrations of BFA; S2 cells carrying genome-edited BFA-hypersensitive mutant Sec71F713Y produced BFA bodies at low concentrations of BFA. These results indicate that Sec71 is the sole BFA target for BFA body formation and controls Golgi stack separation. Finally, we showed that impairment of Sec71 in fly photoreceptors induces BFA body formation with accumulation of both apical and basolateral cargos, resulting in inhibition of polarized transport.