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Primary myeloid cell proteomics and transcriptomics: importance of ß tubulin isotypes for osteoclast function
Author(s) -
David Guérit,
Pauline Marie,
Anne Morel,
Justine Maurin,
Christel Vérollet,
Brigitte Raynaud-Messina,
Serge Urbach,
Anne Blangy
Publication year - 2020
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.239772
Subject(s) - biology , stable isotope labeling by amino acids in cell culture , isotype , podosome , microbiology and biotechnology , myeloid , transcriptome , tubulin , haematopoiesis , immunology , proteomics , gene expression , gene , stem cell , cell , antibody , genetics , microtubule , monoclonal antibody , cytoskeleton
Among hematopoietic cells, osteoclasts (Oc) and immature dendritic cells (Dc) are closely related myeloid cells with distinct functions; Oc participate skeleton maintenance while Dc sample the environment for foreign antigens. Such specificities rely on profound modifications of gene and protein expression during Oc and Dc differentiation. We provide global proteomic and transcriptomic analyses of primary mouse Oc and Dc, based on original SILAC and RNAseq data. We established specific signatures for Oc and Dc including genes and proteins of unknown functions. In particular, we showed that Oc and Dc have the same α and β tubulin isotypes repertoire but that Oc express much more β tubulin isotype Tubb6. In both mouse and human Oc, we demonstrate that elevated expression of Tubb6 in Oc is necessary for correct podosomes organization and thus for the structure of the sealing zone, which sustains the bone resorption apparatus. Hence, lowering Tubb6 expression hindered Oc resorption activity. Overall, we highlight here potential new regulators of Oc and Dc biology and illustrate the functional importance of the tubulin isotype repertoire in the biology of differentiated cells.

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