Open AccessChondrosarcoma-associated gene 1 (CSAG1) maintains the integrity of the mitotic centrosome in cells with defective p53
Author(s) -
Hem Sapkota,
Jonathan D. Wren,
Gary J. Gorbsky
Publication year - 2020
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.239723
Subject(s) - centrosome , biology , mitosis , microbiology and biotechnology , centrosome cycle , spindle apparatus , microtubule , spindle pole body , chromosome segregation , plk1 , function (biology) , genetics , gene , chromosome , cell cycle , cell division , cell
Centrosomes focus microtubules to promote mitotic spindle bipolarity, a critical requirement for balanced chromosome segregation. Comprehensive understanding of centrosome function and regulation requires a complete inventory of components. While many centrosome components have been identified, others may yet remain undiscovered. We have used a bioinformatics approach, based on “guilt by association” expression to identify novel mitotic components among the large group of predicted human proteins that have yet to be functionally characterized. Here we identify Chondrosarcoma-Associated Gene 1 (CSAG1) in maintaining centrosome integrity during mitosis. Depletion of CSAG1 disrupts centrosomes and leads to multipolar spindles more effectively in cells with compromised p53 function. Thus, CSAG1 may reflect a class of “mitotic addiction” genes whose expression is more essential in transformed cells.