Open AccessCCDC102B functions in centrosome linker assembly and centrosome cohesion
Author(s) -
Yuqing Xia,
Ning Huang,
Zhiquan Chen,
Fangyuan Li,
Guiliang Fan,
Dandan Ma,
Jianguo Chen,
Teng Jiang
Publication year - 2018
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.222901
Subject(s) - centrosome , centrosome cycle , biology , microbiology and biotechnology , mitosis , linker , microtubule , microtubule organizing center , interphase , genetics , cell cycle , gene , computer science , operating system
The proteinaceous linker of centrosomes is an important structure that allows the centrosome to function as a single microtubule organizing center (MTOC) of cells in interphase. However, the assembly mechanism of the centrosome linker components remains largely unknown. In this study, we identify CCDC102B as a new centrosome linker protein that is required for maintaining centrosome cohesion. CCDC102B is recruited to the centrosome by C-Nap1 and interacts with the centrosome linker components Rootletin and LRRC45. CCDC102B decorates and facilitates the formation of Rootletin filaments. Furthermore, CCDC102B is phosphorylated by Nek2A and is disassociated from the centrosome at the onset of mitosis. Together, our findings reveal a molecular role for CCDC102B in centrosome cohesion and centrosome linker assembly.