Open AccessTransbilayer phospholipid movement facilitates annexin translocation across membranes
Author(s) -
Sarah E. Stewart,
Avraham Ashkenazi,
Athena Williamson,
David C. Rubinsztein,
Kévin Moreau
Publication year - 2018
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.217034
Subject(s) - phospholipid scramblase , biology , microbiology and biotechnology , extracellular , cytosol , annexin , annexin a2 , phospholipid , membrane , phosphatidylserine , cell membrane , cytoplasm , biochemistry , biophysics , cell , enzyme
Annexins are cytosolic phospholipid-binding proteins that can be found on the outer leaflet of the plasma membrane. The extracellular functions of annexin include modulating fibrinolysis activity and cell migration. Despite having well-described extracellular functions, the mechanism of annexin transport from the cytoplasmic inner leaflet to the extracellular outer leaflet of the plasma membrane remains unclear. Here, we show that the transbilayer movement of phospholipids facilitates the transport of annexins A2 and A5 across membranes in cells and in liposomes. We identified TMEM16F (also known as anoctamin-6, ANO6) as a lipid scramblase required for transport of these annexins to the outer leaflet of the plasma membrane. This work reveals a mechanism for annexin translocation across membranes which depends on plasma membrane phospholipid remodelling.