Open AccessARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments
Author(s) -
Mohamed Bourmoum,
Ricardo Charles,
Audrey Claing
Publication year - 2018
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.216598
Subject(s) - establishment of sister chromatid cohesion , sister chromatids , cohesin , kinetochore , biology , microbiology and biotechnology , separase , metaphase , anaphase , chromatid , chromosome segregation , centromere , spindle checkpoint , mitosis , microtubule , spindle apparatus , genetics , cell division , chromosome , cell cycle , cell , gene
Sister chromatid cohesion, ensured by the protein complex, cohesin, is crucial for the establishment of stable bipolar attachments of chromosomes to the spindle microtubules and their faithful segregation. Here, we demonstrate that the GTPase ARF6 prevents the premature loss of sister chromatid cohesion. During mitosis, ARF6 depleted cells normally completed chromosome congression. However, at the metaphase plate, chromosomes failed to establish stable kinetochore-microtubule attachments because of the impaired cohesion at centromeres. As a result, the spindle assembly checkpoint (SAC) was active and cyclin B ubiquitination and degradation were blocked. Chromosomes/chromatids, in these cells, scattered gradually from the metaphase plate to the two poles of the cell or remained blocked at the metaphase plate for hours. Our study demonstrates that the small GTP-binding protein ARF6 is essential for maintaining centromeric cohesion between sister chromatids, which is necessary for the establishment of stable k-fibres, SAC satisfaction and the anaphase onset.