Open AccessBack localized adenylyl cyclase a mRNA is actively translated in live chemotaxing Dictyostelium cells
Author(s) -
Weiye Wang,
Song Chen,
Satarupa Das,
Wolfgang Losert,
Carole A. Parent
Publication year - 2018
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.216176
Subject(s) - biology , adenylyl cyclase , dictyostelium , microbiology and biotechnology , messenger rna , adcy10 , adcy9 , dictyostelium discoideum , mycetozoa , gene , genetics , signal transduction
Dictyostelium discoideum cells transport adenylyl cyclase A (ACA) containing vesicles to the back of polarized cells to relay exogenous cAMP signals during chemotaxis. Fluorescence in situ hybridization (FISH) experiments showed that ACA mRNA is also asymmetrically distributed at the back of polarized cells. Using the MS2 bacteriophage system, we now visualized the distribution of ACA mRNA in live chemotaxing cells. We found that the ACA mRNA localization is not dependent on the translation of the protein product and requires multiple cis-acting elements within the ACA coding sequence. We show that ACA mRNA is associated with actively translating ribosomes and is transported along microtubules towards the back of cells. By monitoring the recovery of ACA-YFP after photobleach we observed that local translation of ACA-YFP occurs at the back of cells. These data represent a novel functional role for localized translation in the relay of chemotactic signals during chemotaxis.