Open AccessCREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells
Author(s) -
Iris Alejandra García,
Vanina Andrea Torres Demichelis,
Diego L. Viale,
Pablo Di Giusto,
Yulia Ezhova,
Roman Polishchuk,
Luciana Sampieri,
Hernán Martinez,
Elizabeth Sztul,
Cecilia Álvarez
Publication year - 2017
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.211102
Subject(s) - golgi apparatus , biology , secretion , microbiology and biotechnology , effector , transcription factor , secretory pathway , hormone , downregulation and upregulation , endocrinology , biochemistry , gene , endoplasmic reticulum
Many secretory cells increase the synthesis and secretion of cargo proteins in response to specific stimuli. How cells couple increased cargo load with a coordinate rise in secretory capacity to ensure efficient transport is not well understood. We used thyroid cells stimulated with thyrotropin (TSH) to demonstrate a coordinate increase in the production of thyroid-specific cargo proteins and ER-Golgi transport factors, and a parallel expansion of the Golgi complex. TSH also increased expression of the CREB3L1 transcription factor, which alone caused amplified transport factor levels and Golgi enlargement. Furthermore, CREB3L1 potentiated the TSH-induced increase in Golgi volume. A dominant negative CREB3L1 construct hampered the ability of TSH to induce Golgi expansion, implying that this transcription factor contributes to Golgi expansion. Our findings support a model in which CREB3L1 acts as a downstream effector of TSH to regulate the expression of cargo proteins, and simultaneously increase the synthesis of transport factors and the expansion of the Golgi to synchronize the rise in cargo load with the amplified capacity of the secretory pathway.