Open AccessRheb localized on the Golgi membrane activates lysosome-localized mTORC1 at the Golgi-lysosome contact site
Author(s) -
Feike Hao,
Kazuhiko Kondo,
Takashi Itoh,
Sumiko Ikari,
Shigeyuki Nada,
Masato Okada,
Takeshi Noda
Publication year - 2017
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.208017
Subject(s) - golgi apparatus , rheb , lysosome , mtorc1 , microbiology and biotechnology , biology , golgi membrane , biochemistry , endoplasmic reticulum , signal transduction , pi3k/akt/mtor pathway , enzyme
In response to amino acid supply, mTORC1, a master regulator of cell growth, is recruited to the lysosome and activated by a small GTPase, Rheb. However, the intracellular localization of Rheb is controversial. In this study, we showed that a significant portion of Rheb was localized on the Golgi but not on the lysosome. GFP-Rheb could activate mTORC1, even when forced to exclusively localize to the Golgi. Likewise, artificial recruitment of mTORC1 to the Golgi allowed its activation. Accordingly, the Golgi was in contact with the lysosome at the newly described Golgi lysosome contact site (GLCS). The number of GLCSs increased in response to amino acid supply, whereas GLCS perturbation suppressed mTORC1 activation. These results suggest that inter-organelle communication between the Golgi and lysosome is important for mTORC1 regulation and the Golgi-localized Rheb may activate mTORC1 at GLCS.