Open AccessMechanoregulation of clathrin-mediated endocytosis
Author(s) -
Joshua P. Ferguson,
Scott D. Huber,
Nathan M. Willy,
Esra Aygün,
Sevde Goker,
Tuğba Atabey,
Cömert Kural
Publication year - 2017
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.205930
Subject(s) - endocytosis , clathrin , biology , internalization , endocytic cycle , microbiology and biotechnology , receptor mediated endocytosis , actin , cell , biochemistry
We characterized the tension response of clathrin-mediated endocytosis using various cell manipulation methodologies. Elevated tension in a cell hinders clathrin-mediated endocytosis through inhibition of de novo coat initiation, elongation of clathrin coat lifetimes, and reduction of high magnitude growth rates. Actin machinery supplies an inward pulling force necessary for internalization of clathrin coats under high tension. These findings suggest that physical cues cells receive from their microenviroment are major determinants of clathrin-mediated endocytic activity.