Open AccessAtACS8 plays a critical role in the early biosynthesis of ethylene elicited by copper ions in Arabidopsis
Author(s) -
Baogang Zhang,
Haifeng Liu,
Xinhua Ding,
Jiajia Qiu,
Min Zhang,
Chu Zhang
Publication year - 2017
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.202424
Subject(s) - ethylene , biology , biosynthesis , arabidopsis , gene , biochemistry , atp synthase , oxidase test , microbiology and biotechnology , gene expression , enzyme , mutant , catalysis
Copper ions (Cu2+) are required by all living organisms and play important roles in many bactericides and fungicides. We previously reported that Cu2+ could elicit defense responses, which is dependent on the ethylene signaling pathway in Arabidopsis. However, the mechanism by which Cu2+ elicit the biosynthesis of ethylene remains unclear. Here, we show that CuSO4 treatment rapidly increases the production of ethylene. In addition, it up-regulates the expression of several defense-related genes as well as ethylene biosynthesis genes (including S-adenosylmethionine synthase genes, 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS) genes, and ACC oxidase genes. Among these genes, AtACS8 was identified to be essential for the defense response and early ethylene biosynthesis induced by Cu2+. Furthermore, Cu2+-induced AtACS8 expression was determined to depend on the copper-response cis-element (CuRE) in the promoter of AtACS8. Altogether, our study indicates that Cu2+ specifically activate the expression of AtACS8 to promote the early biosynthesis of ethylene that elicits plant immunity in Arabidopsis plants.