MgcRacGAP's SxIP motif tethers Centralspindlin to microtubule plus ends

Centralspindlin, a complex of the kinesin-6 MKLP1 and MgcRacGAP, is required for cytokinesis and cell-cell junctions. During anaphase, Centralspindlin accumulates at overlapping central spindle microtubules and directs contractile ring formation by recruiting the GEF Ect2 to the cell equator to activate RhoA. We found that MgcRacGAP localized to plus ends of equatorial astral microtubules during cytokinesis in Xenopus laevis embryos. How MgcRacGAP is stabilized at microtubule plus ends is unknown. We identified an SxIP motif in X. laevis MgcRacGAP that is conserved with other proteins that bind EB1, a microtubule plus end tracking protein. Mutation of MgcRacGAP's SxIP motif resulted in loss of MgcRacGAP tracking with EB3 on growing microtubule plus ends, abnormal astral microtubule organization, redistribution of MgcRacGAP from the contractile ring to the polar cell cortex, and mislocalization of RhoA and its downstream targets, which together contributed to severe cytokinesis defects. Furthermore, mutation of MgcRacGAP's SxIP motif perturbed adherens junctions. We propose that MgcRacGAP's SxIP motif is functionally important both for its role in regulating adherens junction structure during interphase and for regulating Rho GTPase activity during cytokinesis.