Open AccessCNS macrophages differentially rely on an intronicCsf1renhancer for their development
Author(s) -
David A. D. Munro,
Barry Bradford,
Samanta A. Mariani,
David W. Hampton,
Chris S. Vink,
Siddharthan Chandran,
David Hume,
Clare Pridans,
Josef Priller
Publication year - 2020
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.15
H-Index - 36
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.194449
Subject(s) - choroid plexus , biology , microglia , embryonic stem cell , microbiology and biotechnology , mesenchyme , stromal cell , macrophage , parenchyma , enhancer , central nervous system , anatomy , immunology , neuroscience , embryo , gene , genetics , transcription factor , inflammation , cancer research , in vitro , botany
The central nervous system hosts parenchymal macrophages, known as microglia, and non-parenchymal macrophages, collectively termed border-associated macrophages (BAMs). Microglia, but not BAMs, were reported to be absent in mice lacking a conserved Csf1r enhancer: the fms -intronic regulatory element (FIRE). However, it is unknown whether FIRE deficiency also impacts BAM arrival and/or maintenance . Here, we show that macrophages in the ventricular system of the brain, including Kolmer's epiplexus macrophages, are absent in Csf1r ΔFIRE/ΔFIRE mice. Stromal choroid plexus BAMs are also considerably reduced. During normal development, we demonstrate that intracerebroventricular macrophages arrive from embryonic day 10.5, and can traverse ventricular walls in embryonic slice cultures. In Csf1r ΔFIRE/ΔFIRE embryos, the arrival of both primitive microglia and intracerebroventricular macrophages was eliminated, whereas the arrival of cephalic mesenchyme and stromal choroid plexus BAMs was only partially restricted. Our results provide new insights into the development and regulation of different CNS macrophage populations.