
Establishment of a relationship between blastomere geometry and YAP localisation during compaction
Author(s) -
Christophe Royer,
Karolis Leonavicius,
Annemarie Kip,
Deborah Fortin,
Kirtirupa Nandi,
Anna Vincent,
Céline Jones,
Tim Child,
Kevin Coward,
Chris Graham,
Shankar Srinivas
Publication year - 2020
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.754
H-Index - 325
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.189449
Subject(s) - blastomere , biology , embryo , context (archaeology) , microbiology and biotechnology , hippo signaling pathway , polarity (international relations) , gastrulation , embryogenesis , anatomy , geometry , effector , cell , genetics , paleontology , mathematics
Precise patterning within the three-dimensional context of tissues, organs and embryos implies that cells can sense their relative position. During preimplantation development, outside and inside cells rely on apicobasal polarity and the Hippo pathway to choose their fate. Despite recent findings suggesting that mechanosensing might be central to this process, the relationship between blastomere geometry (i.e. shape and position) and the Hippo pathway effector YAP remains unknown. We used a highly quantitative approach to analyse information on the geometry and YAP localisation of individual blastomeres of mouse and human embryos. We identified the proportion of exposed cell surface area as most closely correlating with the nuclear localisation of YAP. To test this relationship, we developed several hydrogel-based approaches to alter blastomere geometry in cultured embryos. Unbiased clustering analyses of blastomeres from such embryos revealed that this relationship emerged during compaction. Our results therefore pinpoint the time during early embryogenesis when cells acquire the ability to sense changes in geometry and provide a new framework for how cells might integrate signals from different membrane domains to assess their relative position within the embryo.