Open AccessYkt6-dependent endosomal recycling is required for Wnt secretion in the Drosophila wing epithelium
Author(s) -
Karen Linnemannstöns,
Leonie Witte,
Pradhipa Karuna,
Jeanette Kittel,
Adi Danieli,
Denise Madeddu,
Leitsch,
Mona HonemannCapito,
Ferdi Grawe,
Andreas Wodarz,
Julia Christina Gross
Publication year - 2020
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.754
H-Index - 325
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.185421
Subject(s) - endosome , biology , microbiology and biotechnology , wnt signaling pathway , morphogen , secretion , endocytosis , imaginal disc , cytosol , exocytosis , rab , biogenesis , compartment (ship) , drosophila melanogaster , cell , signal transduction , intracellular , genetics , biochemistry , gtpase , oceanography , geology , gene , enzyme
Morphogens are important signalling molecules for tissue development and their secretion requires tight regulation. In the wing imaginal disc of flies, the morphogen Wnt/Wingless is apically presented by the secreting cell and re-internalized before final long-range secretion. Why Wnt molecules undergo these trafficking steps and the nature of the regulatory control within the endosomal compartment remain unclear. Here, we have investigated how Wnts are sorted at the level of endosomes by the versatile v-SNARE Ykt6. Using in vivo genetics, proximity-dependent proteomics and in vitro biochemical analyses, we show that most Ykt6 is present in the cytosol, but can be recruited to de-acidified compartments and recycle Wnts to the plasma membrane via Rab4-positive recycling endosomes. Thus, we propose a molecular mechanism by which producing cells integrate and leverage endocytosis and recycling via Ykt6 to coordinate extracellular Wnt levels.