Open AccessThe subcortical maternal complex protein Nlrp4f is involved in cytoplasmic lattice formation and organelle distribution
Author(s) -
Dandan Qin,
Zheng Gao,
Yi Xiao,
Xiaoxin Zhang,
Hui Ma,
Xingjiang Yu,
Xiaoqing Nie,
Na Fan,
Xiaoqing Wang,
YingChun Ouyang,
QingYuan Sun,
Zhaohong Yi,
Lei Li
Publication year - 2019
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.15
H-Index - 36
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.183616
Subject(s) - biology , organelle , cytoplasm , microbiology and biotechnology , oocyte , embryo
In mammalian oocytes and embryos, the subcortical maternal complex (SCMC) and cytoplasmic lattices (CPLs) are two closely related structures. Their detailed compositions and functions remain largely unclear. Here, we characterized Nlrp4f as a novel component associated with the SCMC and CPLs. Disruption of maternal Nlrp4f leads to decreased fecundity and delayed preimplantation development in the mouse. Lack of Nlrp4f affects organelle distribution in mouse oocytes and early embryos. Depletion of Nlrp4f disrupts CPL formation but does not affect the interactions of other SCMC proteins. Interestingly, the loss of Filia or Tle6, two other SCMC proteins, also disrupts CPL formation in mouse oocytes. Thus, the absence of CPLs and aberrant distribution of organelles in the oocytes disrupted the examined SCMC genes, including previously reported Zbed3, Mater, Floped and Padi6, indicate that the SCMC is required for CPL formation and organelle distribution. Consistent with the SCMC's role in CPL formation, the SCMC forms before CPLs during oogenesis. Together, our results suggest that SCMC protein Nlrp4f is involved in CPL formation and organelle distribution in mouse oocytes.