Open AccessDynamics of activating and repressive histone modifications in Drosophila neural stem cell lineages and brain tumors
Author(s) -
Merve Deniz Abdusselamoglu,
Lisa Landskron,
Sarah K. Bowman,
Elif Eroğlu,
Thomas R Burkard,
Robert E. Kingston,
Juergen A. Knoblich
Publication year - 2019
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.754
H-Index - 325
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.183400
Subject(s) - biology , neuroblast , neural stem cell , lineage (genetic) , epigenetics , histone , psychological repression , cellular differentiation , gene , genetics , drosophila melanogaster , stem cell , microbiology and biotechnology , gene expression , neurogenesis
During central nervous system (CNS) development, spatiotemporal gene expression programs mediate specific lineage decisions to generate neuronal and glial cell types from neural stem cells (NSCs). However, little is known about the epigenetic landscape underlying these highly complex developmental events. Here, we perform ChIP-seq on distinct subtypes of Drosophila FACS- purified neural stem cells (NSCs) and their differentiated progeny to dissect the epigenetic changes accompanying the major lineage decisions in vivo. By analyzing active and repressive histone modifications, we show that stem cell identity genes are silenced during differentiation by loss of their activating marks and not via repressive histone modifications. Our analysis also uncovers a new set of genes specifically required for altering lineage patterns in type II neuroblasts, one of the two main Drosophila NSC identities. Finally, we demonstrate that this subtype specification in NBs, unlike NSC differentiation, requires Polycomb-group (PcG)-mediated repression.