z-logo
open-access-imgOpen Access
Centrosome Aurora A regulates RhoGEF ECT-2 localisation and ensures a single PAR-2 polarity axis in C. elegans embryos
Author(s) -
Sukriti Kapoor,
Sachin Kotak
Publication year - 2019
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.15
H-Index - 36
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.174565
Subject(s) - centrosome , biology , microbiology and biotechnology , polarity (international relations) , cell polarity , caenorhabditis elegans , microtubule , cell , genetics , cell cycle , gene
Proper establishment of cell polarity is essential for development. In the one-cell C. elegans embryo, a centrosome-localised signal provides spatial information for polarity establishment. It is hypothesised that this signal causes local inhibition of the cortical actomyosin network, and breaks symmetry to direct partitioning of the PAR proteins. However, the molecular nature of the centrosomal signal that triggers cortical anisotropy in the actomyosin network to promote polarity establishment remains elusive. Here, we discover that depletion of Aurora A kinase (AIR-1 in C. elegans) causes pronounced cortical contractions on the embryo surface, and this creates more than one PAR-2 polarity axis. This function of AIR-1 appears independent of its role in microtubule nucleation. Importantly, upon AIR-1 depletion, centrosome positioning becomes dispensable in dictating the PAR-2 axis. Moreover, we uncovered that a Rho GEF, ECT-2 acts downstream to AIR-1 in regulating contractility and PAR-2 localisation, and notably, AIR-1 depletion influences ECT-2 cortical localisation. Overall, our study unravels a novel insight whereby an evolutionarily conserved centrosome Aurora A kinase inhibits promiscuous PAR-2 domain formation to ensure singularity in the polarity establishment axis.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here