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Novel initiator caspase reporters uncover unknown features of caspase-activating cells
Author(s) -
Luis Alberto BaenaLópez,
Lewis Arthurton,
Marcus Bischoff,
JeanPaul Vincent,
Cyrille Alexandre,
Reuben McGregor
Publication year - 2018
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.754
H-Index - 325
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.170811
Subject(s) - biology , microbiology and biotechnology , caspase , caspase 2 , transcription factor , caspase 1 , caspase 8 , caspase 3 , apoptosis , computational biology , caspase 9 , genetics , programmed cell death , gene
The caspase-mediated regulation of many cellular processes, including apoptosis, justifies the substantial interest in understanding all of the biological features of these enzymes. To complement functional assays, it is crucial to identify caspase-activating cells in live tissues. Our work describes novel initiator caspase reporters that, for the first time, provide direct information concerning the initial steps of the caspase activation cascade in Drosophila tissues. One of our caspase sensors capitalises on the rapid subcellular localisation change of a fluorescent marker to uncover novel cellular apoptotic events relating to the actin-mediated positioning of the nucleus before cell delamination. The other construct benefits from caspase-induced nuclear translocation of a QF transcription factor. This feature enables the genetic manipulation of caspase-activating cells and reveals the spatiotemporal patterns of initiator caspase activity. Collectively, our sensors offer experimental opportunities not available by using previous reporters and have proven useful to illuminate previously unknown aspects of caspase-dependent processes in apoptotic and non-apoptotic cellular scenarios.

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