Open AccessHeterogeneous fates and dynamic rearrangement of regenerative epidermis-derived cells during zebrafish fin regeneration
Author(s) -
Eri Shibata,
Kazunori Ando,
Emiko Murase,
Koichi Kawakami
Publication year - 2018
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.754
H-Index - 325
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.162016
Subject(s) - biology , epidermis (zoology) , regeneration (biology) , microbiology and biotechnology , zebrafish , population , anatomy , genetics , demography , sociology , gene
The regenerative epidermis (RE) is a specialized tissue that plays an essential role in tissue regeneration. However, the fate of the RE during and after regeneration is unknown. In this study, we performed Cre-loxP-mediated cell fate tracking and revealed the fates of major population of regenerative epidermis cells that express fibronectin 1b (fn1b) during zebrafish fin regeneration. Our study showed that these RE cells are mainly recruited from the inter-ray epidermis, and that they follow heterogeneous cell fates. Early recruited cells contribute to initial wound healing and soon disappear by apoptosis, while the later recruited cells contribute to the regenerated epidermis. Intriguingly, many of these cells were also expelled from the regenerated tissue by a dynamic caudal movement of the epidermis over time, and in turn the loss of epidermal cells was replenished by a global self-replication of basal and suprabasal cells in fin. De-differentiation of non-basal epidermal cells into the basal epidermal cells did not occur during regeneration. Overall, our study revealed heterogeneous fates of RE cells and a dynamic rearrangement of the epidermis during and after regeneration.