Open AccessPDGFRα / PDGFRβ signaling balance modulates progenitor cell differentiation into white and beige adipocytes
Author(s) -
ZhanGuo Gao,
Alexes C. Daquinag,
Fei Su,
Brad Snyder,
Mikhail G. Kolonin
Publication year - 2017
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.754
H-Index - 325
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.155861
Subject(s) - biology , adipogenesis , adipose tissue , adipocyte , platelet derived growth factor receptor , progenitor cell , white adipose tissue , stromal cell , endocrinology , microbiology and biotechnology , medicine , stem cell , receptor , cancer research , growth factor , genetics
Relative abundance of thermogenic beige adipocytes and lipid-storing white adipocytes in adipose tissue underlie its metabolic activity. The roles of adipocyte progenitor cells, which express PDGFRα or PDGFRβ, in adipose tissue function have remained unclear. Here, by defining the developmental timing of PDGFRα and PDGFRβ expression in mouse subcutaneous and visceral adipose depots, we uncover depot-specificity of preadipocyte delineation. We demonstrate that PDGFRα expression precedes PDGFRβ expression in all subcutaneous but only in a fraction of visceral adipose stromal cells. We show that high fat diet feeding or thermoneutrality in early postnatal development can induce PDGFRβ+ lineage recruitment to generate white adipocytes. In contrast, the contribution of PDGFRβ+ lineage to beige adipocytes is minimal. We provide evidence that human adipose tissue also contains distinct progenitor populations differentiating into beige or white adipocytes depending on PDGFRβ expression. Based on PDGFRα or PDGFRβ deletion and ectopic expression experiments, we conclude that the PDGFRα / PDGFRβ signaling balance determines progenitor commitment to beige or white adipogenesis, respectively. Our study suggests that adipocyte lineage specification and metabolism can be modulated through PDGFR signaling.