
High efficiency non-mosaic CRISPR mediated knock-in and mutations in F0 Xenopus
Author(s) -
Yetki Aslan,
Emmanuel Tadjuidje,
Aaron M. Zorn,
SangWook Cha
Publication year - 2017
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.15
H-Index - 36
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.152967
Subject(s) - biology , crispr , genome editing , genetics , homology directed repair , homologous recombination , indel , gene , gene targeting , genome , xenopus , germline , transcription activator like effector nuclease , mutation , mutant , computational biology , dna repair , dna mismatch repair , genotype , single nucleotide polymorphism
The revolution in CRISPR-mediated genome editing has enabled the mutation and insertion of virtually any DNA sequence, particularly in cell culture where selection can be used to recover relatively rare homologous recombination events. The efficient use of this technology in animal models still presents a number of challenges including the time to establish mutant lines, mosaic gene editing in founder animals, and low homologous recombination rates. Here we report a method for CRISPR-mediated genome editing in Xenopus oocytes with homology-dependent repair (HDR) that provides efficient non-mosaic targeted insertion of small DNA fragments of 40-50 nucleotides, in 4.4 to 25.7% of F0 tadpoles, with germline transmission. For both for CRISPR/Cas9-mediated HDR gene editing and indel mutation, the gene-edited F0 embryos are uniformly heterozygous, consistent with a mutation in only the maternal genome. In addition to efficient tagging of proteins in vivo, this HDR methodology will allow researchers to create patient-specific mutations for human disease modeling in Xenopus.