Open AccessEndothelial cells respond to the direction of mechanical stimuli through SMAD signaling to regulate coronary artery size
Author(s) -
Aruna Poduri,
Andrew Chang,
Brian Raftrey,
Siyeon Rhee,
Mike V. Van,
Kristy RedHorse
Publication year - 2017
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.754
H-Index - 325
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.150904
Subject(s) - mechanotransduction , biology , smad , blood flow , microbiology and biotechnology , artery , coronary arteries , signal transduction , medicine , endothelium , phenotype , anatomy , endocrinology , genetics , gene
How mechanotransduction intersects with chemical and transcriptional factors to shape organogenesis is an important question in developmental biology. This is particularly relevant to the cardiovascular system, which uses mechanical signals from flowing blood to stimulate cytoskeletal and transcriptional responses that form a highly efficient vascular network. Using this system, artery size and structure are tightly regulated, but the underlying mechanisms are poorly understood. Here, we demonstrate that deletion of Smad4 increased the diameter of coronary arteries during embryonic development, a phenotype that followed the initiation of blood flow. At the same time, the BMP signal transducers SMAD1/5/8 were activated in developing coronary arteries. In a culture model of blood flow-induced shear stress, coronary artery endothelial cells failed to align when either BMPs were inhibited or Smad4 was depleted. In contrast to control cells, Smad4-deficient cells did not migrate against the direction of shear stress and increased proliferation rates specifically under flow. Similar alterations were seen in coronary arteries in vivo. Thus, endothelial cells perceive the direction of blood flow and respond through SMAD signaling to regulate artery size.