Open AccessBMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells in mice
Author(s) -
Jifan Feng,
Junjun Jing,
Jingyuan Li,
Hu Zhao,
Vasu Punj,
T. Zhang,
Jian Xu,
Yang Chai
Publication year - 2017
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.754
H-Index - 325
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.150136
Subject(s) - biology , mesenchymal stem cell , microbiology and biotechnology , stem cell , transcription factor , genetics , gene
Signaling pathways are used reiteratively in different developmental processes yet produce distinct cell fates through specific downstream transcription factors. In this study, we used tooth root development as a model to investigate how the BMP signaling pathway regulates transcriptional complexes to direct the fate determination of multipotent mesenchymal stem cells (MSCs). We first identified the MSC population supporting mouse molar root growth as Gli1+ cells. Using a Gli1-mediated transgenic animal model, our results provide the first in vivo evidence that BMP signaling activity is required for the odontogenic differentiation of MSCs. Specifically, we identified transcription factors, Pax9, Klf4, Satb2, and Lhx8, that are downstream of BMP signaling and are expressed in a spatially restricted pattern potentially involved in determining distinct cellular identities within the dental mesenchyme. Finally, we found that overactivation of one key transcription factor, Klf4, associated with the odontogenic region, promotes odontogenic differentiation of MSCs. Collectively, our results demonstrate the functional significance of BMP signaling in regulating MSC fate during root development and shed light on how BMP signaling can achieve functional specificity in regulating diverse organ development.