Open AccessA single morphogenetic field gives rise to two retina primordia under the influence of the prechordal plate
Author(s) -
Hua Shun Li,
Christopher Tierney,
Lijun Wen,
Jane Y. Wu,
Yi Rao
Publication year - 1997
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.754
H-Index - 325
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.124.3.603
Subject(s) - biology , retina , xenopus , anatomy , primordium , neuroectoderm , microbiology and biotechnology , mesoderm , optic vesicle , zebrafish , neural plate , eye development , neuroscience , genetics , gene , embryonic stem cell , phenotype
Two bilaterally symmetric eyes arise from the anterior neural plate in vertebrate embryos. An interesting question is whether both eyes share a common developmental origin or they originate separately. We report here that the expression pattern of a new gene ET reveals that there is a single retina field which resolves into two separate primordia, a suggestion supported by the expression pattern of the Xenopus Pax-6 gene. Lineage tracing experiments demonstrate that retina field resolution is not due to migration of cells in the median region to the lateral parts of the field. Removal of the prechordal mesoderm led to formation of a single retina both in chick embryos and in Xenopus explants. Transplantation experiments in chick embryos indicate that the prechordal plate is able to suppress Pax-6 expression. Our results provide direct evidence for the existence of a single retina field, indicate that the retina field is resolved by suppression of retina formation in the median region of the field, and demonstrate that the prechordal plate plays a primary signaling role in retina field resolution.