Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana | Zendy

Sadia Hamera | Zendy; Youngsheng Yan | Zendy; Xiaoguang Song | Zendy; Safee Ullah Chaudhary | Zendy; Iram Murtaza | Zendy; Lei Su | Zendy; Muhammad Tariq | Zendy; Xiaoying Chen | Zendy; Rongxiang Fang | Zendy

Open Access

Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana

Author(s) -

Sadia Hamera,

Youngsheng Yan,

Xiaoguang Song,

Safee Ullah Chaudhary,

Iram Murtaza,

Lei Su,

Muhammad Tariq,

Xiaoying Chen,

Rongxiang Fang

Publication year - 2016

Publication title -

biology open

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.936

H-Index - 41

ISSN - 2046-6390

DOI - 10.1242/bio.017244

Subject(s) - biology , cucumber mosaic virus , arabidopsis thaliana , suppressor , arabidopsis , methylation , virology , virus , microbiology and biotechnology , plant virus , mosaic , botany , genetics , gene , mutant , history , archaeology

The Cucumber mosaic virus (CMV) suppressor 2b co-localizes with AGO4 in cytoplasmic and nuclear fractions of Arabidopsis thaliana Biochemical fractionation of A. thaliana cellular extracts revealed that 2b and AGO4 coexist in multiple size exclusions. 2b transgenic A. thaliana exhibited an enhanced accumulation of 24nt siRNAs from flowering wageningen (FWA) and other heterochromatic loci. These plants also exhibited hypo-methylation of an endogenous- as well as transgene-FWA promoter at non-CG sites. In corroboration, both transgenic 2b and CMV infection affected the regulation of transposons which mimics the ago4 phenotype. In conclusion, 2b perturbs plant defense by interfering with AGO4-regulated transcriptional gene silencing.

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