Open AccessInhibition of KCC2 in Mouse Spinal Cord Neurons Leads to Hypersensitivity to Thermal Stimulation
Author(s) -
Thomas M. Austin,
Eric Delpire
Publication year - 2011
Publication title -
anesthesia and analgesia/anesthesia and analgesia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.404
H-Index - 201
eISSN - 1526-7598
pISSN - 0003-2999
DOI - 10.1213/ane.0b013e31822e0a5d
Subject(s) - bumetanide , medicine , nociception , stimulation , spinal cord , cotransporter , neuropathic pain , pharmacology , anesthesia , endocrinology , receptor , chemistry , organic chemistry , psychiatry , sodium
KCC2, a neuronal-specific K-Cl cotransporter, is involved in pain perception physiology through its effects on postsynaptic inhibition in spinal cord neurons. We injected a newly identified, highly potent and selective inhibitor of KCC2 (D4), an inactive structural variant (D4.14), and the Na-K-2Cl cotransporter (NKCC1) inhibitor, bumetanide, into the intrathecal space of mice to measure their effect on heat-evoked nociceptive responses.