Open AccessFlow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study
Author(s) -
Miles Berger,
David M Murdoch,
Janet Staats,
Cliburn Chan,
John P. Thomas,
Grant E. Garrigues,
Jeffrey N. Browndyke,
Mary Cooter,
Quintin J. Quiñones,
Joseph P. Mathew,
Kent J. Weinhold,
Madco-Pc Study Team
Publication year - 2019
Publication title -
anesthesia and analgesia/anesthesia and analgesia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.404
H-Index - 201
eISSN - 1526-7598
pISSN - 0003-2999
DOI - 10.1213/ane.0000000000004179
Subject(s) - medicine , cerebrospinal fluid , monocyte , flow cytometry , neurocognitive , postoperative cognitive dysfunction , lymphocyte , anesthesia , immunology , cognition , pathology , psychiatry
Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.