Open AccessOxidative stress parameters in different systemic rheumatic diseases
Author(s) -
Firuzi Omidreza,
Fuksa Leoš,
Spadaro Chiara,
Boušovà Iva,
Riccieri Valeria,
Spadaro Antonio,
Petrucci Rita,
Marrosu Giancarlo,
Saso Luciano
Publication year - 2006
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1211/jpp.58.7.0010
Subject(s) - oxidative stress , antioxidant , pathogenesis , rheumatoid arthritis , medicine , endocrinology , ferrous , antioxidant capacity , chemistry , biochemistry , organic chemistry
The involvement of oxidative stress in the pathogenesis of rheumatic disorders, such as systemic sclerosis (SSc) and chronic polyarthritides, has been suggested yet not thoroughly verified experimentally. We analysed 4 plasmatic parameters of oxidative stress in patients with SSc (n = 17), psoriatic arthritis (PsA) (n = 10) and rheumatoid arthritis (RA) (n = 9) compared with healthy subjects (n = 22). The biomarkers were: total antioxidant capacity (TAC) measured by ferric reducing antioxidant power (FRAP) method, hydroperoxides determined by ferrous ion oxidation in presence of xylenol orange (FOX) method and sulfhydryl and carbonyl groups assessed by spectrophotometric assays. The results showed significantly increased hydroperoxides in SSc, PsA and RA (3.97 ± 2.25, 4.87 ± 2.18 and 5.13 ± 2.36 μmol L −1 , respectively) compared with the control group, (2.31 ± 1.40 μmol L −1 ; P < 0.05). Sulfhydryls were significantly lower in SSc (0.466 ± 0.081 mmol L −1 ), PsA (0.477 ± 0.059 mmol L −1 ) and RA (0.439 ± 0.065 mmol L −1 ) compared with the control group) (0.547 ± 0.066 mmolL −1 ; P < 0.05). TAC in all three diseases showed no difference in comparison with controls. Carbonyls were significantly higher in RA than in the control group (32.1 ± 42 vs 2.21 ± 1.0 nmol (mg protein) −1 ; P < 0.05). The obtained data indicate augmented free radical‐mediated injury in these rheumatic diseases and suggest a role for the use of antioxidants in mediated prevention and treatment of these pathologies.