The Chicken c-erbA α-Product Induces Expression of Thyroid Hormone-Responsive Genes in 3,5,3′-Triiodothyronine Receptor- Deficient Rat Hepatoma Cells | Zendy

Alberto Múñoz | Zendy; Wolfgang Höppner | Zendy; Jan Sap | Zendy; Ged Brady | Zendy; Kristiordström | Zendy; Hans Seitz | Zendy; Björn Vennström | Zendy

Open Access

The Chicken c-erbA α-Product Induces Expression of Thyroid Hormone-Responsive Genes in 3,5,3′-Triiodothyronine Receptor- Deficient Rat Hepatoma Cells

Author(s) -

Alberto Múñoz,

Wolfgang Höppner,

Jan Sap,

Ged Brady,

Kristiordström,

Hans Seitz,

Björn Vennström

Publication year - 1990

Publication title -

molecular endocrinology

Language(s) - English

Resource type - Journals

eISSN - 1944-9917

pISSN - 0888-8809

DOI - 10.1210/mend-4-2-312

Subject(s) - biology , thyroid hormone receptor , thyroid hormone receptor beta , thyroid hormone receptor alpha , triiodothyronine , thyroid , hormone , microbiology and biotechnology , nuclear receptor , gene product , hormone receptor , gene expression , medicine , gene , endocrinology , biochemistry , transcription factor , genetics , cancer , breast cancer

To determine the capacity of the chicken c-erbA (cTR-alpha) gene product in regulating expression of known thyroid hormone-responsive genes, both the cTR-alpha and the viral v-erbA genes were expressed in FAO cells, a rat hepatoma cell line defective for functional thyroid hormone receptors. Upon nuclear expression of the cTR-alpha protein the cells become responsive to thyroid hormone, as detected by expression of a number of genes (malic enzyme, phosphoenolpyruvate carboxykinase, and Na+/K(+)-ATPase) reported to be indirectly induced by the hormone in vivo. In addition, our data show that the c-erbA product directly activates the Moloney murine leukemia virus promoter in a ligand-dependent manner. The data show that the chicken c-erbA-alpha protein can modulate the expression of rat genes under either direct or indirect control by thyroid hormone.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research