Open AccessMultiplex Gene Profiling of Cell-Free DNA in Patients With Metastatic Melanoma for Monitoring Disease
Author(s) -
Selena Y. Lin,
Sharon K. Huang,
Kelly Huynh,
Matthew P. Salomon,
Shu-Ching Chang,
Diego M. Marzese,
Richard B. Lanman,
AmirAli Talasaz,
Dave S. B. Hoon
Publication year - 2018
Publication title -
jco precision oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.405
H-Index - 22
ISSN - 2473-4284
DOI - 10.1200/po.17.00225
Subject(s) - concordance , oncology , medicine , cohort , melanoma , cell free fetal dna , lymph node , disease , metastasis , cancer , pathology , biology , cancer research , genetics , pregnancy , fetus , prenatal diagnosis
Hotspot blood cell-free DNA (cfDNA) biomarker assays have limited utility in profiling tumor heterogeneity and burden and in capturing regional metastasis with low disease burden in patients with melanoma. We investigated the utility of a sensitive 54-cancer gene digital next-generation sequencing approach targeting blood cfDNA single nucleotide variants (SNVs) and copy number amplification for monitoring disease in patients with melanoma with regional or distant organ metastasis (DOM).