Open AccessCorrelation Between MYCN Gene Status and MYCN Protein Expression in Neuroblastoma: A Pilot Study To Propose the Use of MYCN Immunohistochemistry in Limited-Resource Areas
Author(s) -
Teresa Santiago,
Nidale Tarek,
Fouad Boulos,
Caleb Hayes,
Sima Jeha,
Susana C. Raimondi,
Carlos RodríguezGalindo
Publication year - 2019
Publication title -
journal of global oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.002
H-Index - 17
ISSN - 2378-9506
DOI - 10.1200/jgo.19.00135
Subject(s) - neuroblastoma , immunohistochemistry , medicine , gene , gene duplication , fluorescence in situ hybridization , oncology , cancer research , gene expression , pathology , biology , genetics , cell culture , chromosome
The most significant adverse risk factor for neuroblastoma (NB) is MYCN gene amplification, which strongly associates with high-risk disease. Fluorescent in situ hybridization (FISH) is considered the best method to evaluate MYCN gene status. However, it requires a laboratory that can perform highly complex testing, specialized personnel, and costly reagents. Herein, we aimed to investigate the feasibility of using immunohistochemistry (IHC) to detect MYCN protein expression in lieu of FISH, a strategy potentially useful in areas with limited resources.