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Fragments of Noncoding RNA in Plasma of Human Blood
Author(s) -
Semenov Dmitry V.,
Baryakin Dmitry N.,
Kamynina Tatyana P.,
Kuligina Elena V.,
Richter Vladimir A.
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1448.030
Subject(s) - rna , complementary dna , biology , long non coding rna , reverse transcriptase , microbiology and biotechnology , non coding rna , adapter (computing) , transcription (linguistics) , computational biology , genetics , gene , electrical engineering , engineering , linguistics , philosophy
Mammalian extracellular fluids, such as plasma of blood or plasma of milk, contain cell‐free RNAs. Fragments of ubiquitously expressed mRNAs as well as tumor‐specific and viral RNAs have been previously revealed in human plasmas by template‐defined RT‐PCR. In the present work we aimed to detect major forms of human blood plasma RNAs or to reveal new forms by using two approaches which were independent of context of RNA templates. The first approach was based on ligation of total plasma RNAs with adapter‐oligoribonucleotides, reverse transcription of RNA‐constructs, and amplification and cloning of cDNA. The second EST‐like approach involved reverse transcription of RNAs with 3′‐N6 degenerate primer, ligation of cDNA with dsDNA‐adapter, amplification and cloning. Using these approaches we determined tens of tiny RNAs of human plasma and identified several long (>100 nt) RNAs. Most of tiny RNAs could be attributed to the fragments of rRNAs, whereas longer forms are representatives of noncoding RNAs.