Premium
Phenotype and mRNA Expression of Syncytiotrophoblast Microparticles Isolated from Human Placenta
Author(s) -
Kumpel Belinda,
King MayJean,
Sooranna Suren,
Jackson Dave,
Eastlake Jane,
Cheng Rong,
Johnson Mark
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1448.017
Subject(s) - syncytiotrophoblast , placenta , phenotype , human placenta , messenger rna , microbiology and biotechnology , chemistry , andrology , fetus , biology , pregnancy , gene , genetics , medicine , biochemistry
Fetal and neonatal alloimmune thromboctyopenia due to maternal human platelet antigen (HPA)‐1a antibodies affects primigravidas. Immunization must occur early in pregnancy before fetal platelets enter maternal blood via fetomaternal hemorrhage. The HPA‐1a antigen is located on platelet glycoprotein (GP)IIIa (CD61, β3 integrin), which is also present on the placental syncytiotrophoblast (ST) and in direct contact with maternal blood. Since ST debris is shed into maternal blood during pregnancy, this material might be immunogenic in vivo . For experimental purposes, we prepared and characterized ST microparticles (STMPs) in vitro from term placentas. Phenotype analysis by flow cytometry and Western blotting showed that STMP expressed more placental alkaline phosphatase (PLAP) than GPIIIa. Quantitative real‐time PCR demonstrated expression of human placental lactogen (HPL), human chorionic gonadotrophin (HCG), and GPIIIa by STMP, in the order HPL > HCG > GPIIIa. PLAP, HPL, and HCG are trophoblast‐specific proteins. These STMPs may be a useful model for studying the natural ST debris in plasma of pregnant women.