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Developing a Novel Model System to Target Insulin‐Reactive CD8 T Cells
Author(s) -
Scott Gwen S.,
Fishman Sigal,
Margalit Alon,
Siew L. Khai,
Chapman Stephen,
Wen Li,
Gross Gideon,
Wong F. Susan
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1447.040
Subject(s) - cytotoxic t cell , cd8 , insulin , cytotoxicity , immunology , interleukin 21 , chemistry , biology , antigen , in vitro , endocrinology , biochemistry
CD8 T cells play an important role in autoimmune diabetes development, and therefore removing these cells may protect against disease. To test this, we designed a novel method using engineered cells (InsCD3‐ζ) to target insulin‐specific CD8 T cells. Insulin‐reactive target cells were cultured with InsCD3‐ζ CD8 T cells and cytotoxicity was assessed. Activated, but not naïve, InsCD3‐ζ CD8 T cells readily killed insulin‐reactive target CD8 T cells. This approach to immunotarget relevant pathogenic CD8 T cells may be a therapeutic option to delay or prevent type 1 diabetes.