Premium
Hedgehog Signaling during Expansion of Human Pancreatic Islet‐Derived Precursors
Author(s) -
Gallo Rita,
Grieco Fabio Arturo,
Marselli Lorella,
Ferretti Elisabetta,
Gulino Alberto,
Marchetti Piero,
Dotta Francesco
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1447.024
Subject(s) - cyclopamine , hedgehog signaling pathway , hedgehog , gli1 , microbiology and biotechnology , mesenchymal stem cell , islet , chemistry , signal transduction , pancreas , biology , cancer research , endocrinology , insulin
A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet‐derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin‐secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Gli1 expression, and that a dose‐dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom