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The Distribution of Adiponectin Receptors on Human Peripheral Blood Mononuclear Cells
Author(s) -
Pang Terence T.L.,
Narendran Parth
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1447.021
Subject(s) - peripheral blood mononuclear cell , adiponectin , flow cytometry , receptor , adiponectin receptor 1 , endocrinology , medicine , chemistry , peripheral , insulin resistance , immunology , biology , diabetes mellitus , in vitro , biochemistry
Adiponectin, an adipocytokine with anti‐inflammatory and insulin‐sensitizing properties, may provide a mechanism by which insulin resistance accelerates autoimmunity in type 1 diabetes (T1D). Its actions are mediated by two receptors, adiponectin receptors 1 (ADIPOR1) and 2 (ADIPOR2). In this study, we measured their distribution on human peripheral mononuclear cells by flow cytometry. ADIPOR1 is present approximately on 1% of T cells, 93% of monocytes, 47% of B cells, and 21% of NK cells ( P < 0.01 for difference between subsets). The distribution of ADIPOR2 was found to be similar ( r = 0.992, P < 0.01), and staining could be blocked in an antigen‐specific manner. We were also able to confirm our finding at an RNA level by PCR using sequence‐specific primers. Our data are consistent with an immunoregulatory role for adiponectin in T1D.