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Human Regulatory CD8 + T Cells
Author(s) -
Ablamunits Vitaly,
Bisikirska Brygida C.,
Herold Kevan C.
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1447.000
Subject(s) - chemistry , physics
Administration of a humanized monoclonal anti‐CD3 antibody (mAb) to patients with type 1 diabetes (T1D) increases their C‐peptide responses and the CD8/CD4 ratio. Incubation of human peripheral blood mononuclear cells (PBMC) with mAb in vitro has been shown to induce CD8 + regulatory T cells (Tregs) capable of inhibiting proliferation of CD4 + T cells. We hypothesized that CD8 + Tregs function through secretion of cytokines. To test that possibility, we generated CD8 + Tregs, sorted them by FACS, incubated them with syngeneic CD8‐depleted PBMC in the presence of staphylococcal enterotoxin B (SEB), and measured proliferation of T cells and cytokines. Using neutralizing anti‐cytokine mAbs, we show that the inhibitory effect of CD8 + Tregs could be partially alleviated by anti‐CCL‐4, anti‐TNF, and to a lesser extent anti‐IL2, suggesting that these cytokines contribute to CD8 + Treg function.