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Analysis of Single, Purified Inclusions as a Novel Approach to Understand Methamphetamine Neurotoxicity
Author(s) -
Lenzi Paola,
Fulceri Federica,
Lazzeri Gloria,
Casini Alessandro,
Ruggieri Stefano,
Paparelli Antonio,
Fornai Francesco
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1432.014
Subject(s) - methamphetamine , neurotoxicity , neuroscience , in vitro , dopamine , intracellular , in vivo , meth , chemistry , biology , pharmacology , biochemistry , toxicity , microbiology and biotechnology , monomer , organic chemistry , acrylate , polymer
A variety of neurodegenerative diseases leading to movement disorders such as Parkinson's disease (PD) are characterized by neuronal inclusions. Despite evidence of the presence of these intrusions, these intracellular bodies have been poorly investigated because of the technical limits of reproducing them in experimental models and the difficulties in isolating these ultrastuctures. Here, we describe a simple method for the isolation of single, purified inclusion bodies using immunomagnetic separation. We profited from the high number and maturation stage of inclusions produced in vitro by methamphetamine (METH) in cultured PC12 cells; in fact, this experimental condition is highly reproducible and has a limited number of experimental variables, while it is predictive of what is described in vivo in dopamine neurons.