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Two‐photon Excitation Fluorescence Bioassays
Author(s) -
Hänninen Pekka,
Soukka Jori,
Soini Juhani T.
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1430.040
Subject(s) - excitation , two photon excitation microscopy , fluorescence , microscopy , photon , excitation wavelength , optics , wavelength , resolution (logic) , fluorescence microscope , bioassay , physics , materials science , chemistry , biology , computer science , quantum mechanics , artificial intelligence , genetics
Application of two‐photon excitation of fluorescence in microscopy is one of the major discoveries of the “renaissance” of light microscopy that started in the 1980s. The technique derives its advantages from the biologically “smooth” wavelength of the excitation light and the confinement of the excitation. Difficult, and seemingly nontransparent, samples may be imaged with the technique with good resolution. Although the bioresearch has been concentrating mostly on the positive properties of the technique for imaging, the same properties may be applied successfully to nonimaging bioassays. This article focuses on the development path of two‐photon excitation–based assay system.