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Toward Improved Biochips Based on Rolling Circle Amplification—Influences of the Microenvironment on the Fluorescence Properties of Labeled DNA Oligonucleotides
Author(s) -
MayerEnthart Elke,
Sialelli Julien,
Rurack Knut,
ReschGenger Ute,
Köster Daniela,
Seitz Harald
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1430.022
Subject(s) - biochip , oligonucleotide , rolling circle replication , dna , fluorescence , chemistry , combinatorial chemistry , biophysics , computational biology , nanotechnology , microbiology and biotechnology , biochemistry , materials science , biology , physics , dna replication , optics
Microarrays have become an increasingly important tool for biotechnology and molecular diagnostics. Despite many advantages, their sensitivity is still insufficient for such tasks as the analysis of small sample quantities and for the detection of alterations in gene expression of low‐abundance genes. Accordingly, amplification strategies are necessary. Approaches to amplify the signal intensity include the increase of the number of dye molecules per target through either particle labels or rolling circle amplification, as used for this study.