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Fluorescence Measurements on Functionalized Polymer Surfaces—Problems and Troubleshooting
Author(s) -
Hoffmann Katrin,
Mix Renate,
ReschGenger Ute,
Friedrich Joerg F.
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1430.015
Subject(s) - troubleshooting , fluorescence , chromogenic , polymer , nanotechnology , adsorption , chemistry , polypropylene , characterization (materials science) , combinatorial chemistry , computer science , materials science , chromatography , organic chemistry , optics , physics , operating system
Plasma‐chemically tailor‐made polymer surfaces are of ever‐increasing importance to control surface properties in material science, as well as for (bio)analytical and biomedical applications. For the characterization of such systems, sensitive fluorescence techniques are attractive tools. To underline the potential and drawbacks of these strategies, this article addresses different problems that complicate fluorometric analysis. To overcome some of these limitations, such as nonspecific adsorption of unreacted fluorescent probes, we discuss potential troubleshooting, including the use of a chromogenic and fluorogenic pyrylium dye for the detection of amino functionalities at polypropylene surfaces.