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Lanthanide Bimetallic Helicates for in Vitro Imaging and Sensing
Author(s) -
Bünzli JeanClaude G.,
Chauvin AnneSophie,
Vandevyver Caroline D.B.,
Bo Song,
Comby Steve
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1430.010
Subject(s) - bimetallic strip , lanthanide , in vitro , chemistry , biophysics , biochemistry , biology , organic chemistry , catalysis , ion
As the need for targeting luminescent biolabels increases, for mapping selected analytes, imaging of cells and organs, and tracking in cellulo processes, lanthanide bimetallic helicates are emerging as versatile bioprobes. The wrapping of three ligand strands around two metallic centers by self‐assembly affords robust molecular edifices with tunable chemical and photophysical properties. In addition, heterometallic helical chelates can be assembled leading to bioprobes with inherent chiral properties. In this paper, we review the literature demonstrating that neutral [Ln 2 (L CX ) 3 ] ( x = 1–3) helicates represent a viable alternative to existing chelating agents for bio‐analyses, while featuring specific enhanced properties. These bimetallic chelates self‐assemble in water, and at physiological pH the 2:3 (Ln:L CX ) complex is by far the dominant species, conditional stability constants logβ 23 being in the range 23–30. The metal ions are 9‐coordinate and lie in sites with slightly distorted D 3 symmetry. Efficient protection from water interaction by the tightly wrapped ligand strands results in sizeable photophysical properties, with quantum yields up to 24% for Eu III and 11% for Tb III , while the luminescence of several other visible and/or near‐infrared emitting Ln III ions is also sensitized. Noncytotoxicity for all the helicates is established for several living cell lines including HeLa, HaCat, MCF‐7, 5D10, and Jurkat. We present new data pertaining to the live cell imaging ability of [Eu 2 (L C1 ) 3 ] and compare the three systems with x = 1–3 with respect to thermodynamic stability, photophysics, cell‐permeation ability, and targeting capability for sensing in cellulo processes. Prospects of derivatization for characterizing specific biological interactions are discussed.