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Toward Prevention of Cowdriosis
Author(s) -
Pedregal Andreia,
De Sousa Daniel Ribeiro,
Nguyen Hoang Nam,
Das Neves Eane Agapito,
Lowagie Serge,
Marique Thierry,
Kagye Nathalie,
Guerra Ines Teixeira,
Kamba Yvette,
Totte Philippe,
Vachiéry Nathalie,
Lefrançois Thierry,
Martinez Dominique,
Yourassowsky Catherine,
Callens Natacha,
Monnom Olivier,
Dubois Frank,
Wérenne John
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1428.091
Subject(s) - microcarrier , ehrlichia , virology , biology , microbiology and biotechnology , cell culture , chemistry , genetics , tick
Mass production of Ehrlichia ruminantium variants from different regions of sub‐Saharan Africa is one of the difficulties that must be overcome in producing a heartwater vaccine. Vaccine productivity can be limited by endogenous induction of interferon (IFN), which inhibits the propagation of Ehrlichia ruminantium (ER) in cell culture. Different kinds of endothelial cells, in which ER multiply efficiently, could be grown in a scalable way in VueLife Teflon bags on Cytodex 3 microcarriers where bead‐to‐bead transfer of cells occurs. The digital holographic microscope designed at the Université Libre de Bruxelles allows detection of the most appropriate time to harvest intracellular microorganisms for vaccine production.