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Lethal Activity of Two Bacillus thuringiensis Strains against Haemonchus contortus Histotropic Larvae
Author(s) -
Linares Ismael Hernández,
Arellano María Eugenia López,
De Gives Pedro Mendoza,
Hernández Enrique Liébano,
De La Parra Alejandra Bravo
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1428.077
Subject(s) - haemonchus contortus , bacillus thuringiensis , larva , biology , microbiology and biotechnology , veterinary medicine , bacteria , ecology , medicine , genetics
The in vitro lethal activity of two Bacillus thuringiensis strains (IB‐16 and IB‐61) against Haemonchus contortus histotropic fourth stage larvae (L 4 ) was evaluated. Each B. thuringiensis strain was used as soluble proteins at different concentrations. In addition, the in vitro lethal dose (LD 50 ) of each strain was determined against H. contortus L 4 at different times. Data were analyzed by ANOVA and Tukey tests. Less than 50% mortality was observed at 50 and 100 μg/mL during all bioassays. More than 50% mortality was observed at 200 μg/mL at 3 and 5 days after interaction with IB‐16 and IB‐61, respectively. A 100% mortality was observed with IB‐16 on day 5. The LD 50 with IB‐16 was 248 μg/mL on day 3, and IB‐61 showed an LD 50 at 227 μg/mL on the day 5. Both toxins may have potential as biocontrol agents.
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